Introduction to Digital Bioimage Analysis

24-27 September 2019 | i3S - Porto

Contents:

Principles, techniques and ethics of digital bioimage processing.

Basic commands of ImageJ/Fiji software.

Working with multidimensional (2D, 3D, time-lapse, position) bioimages.

Enhancement and processing of images. Extract quantitative numerical data.

Design of simple workflows for automation of image analysis with ImageJ macros.

Introduction to Cellprofiler.

Participants are invited to submit in advance an analysis problem/data of their interest that might be addressed in the course as an individual mini-project (with the help of the tutors).

Requirements: The participant should bring a personal laptop for the sessions.

Registration:

Early bird | Until 31 July 2019

i3S members - 100 € | Payment deadline: 2 August 2019

External participants - 150 € | Payment deadline: 2 August 2019

For more information and registration: https://www.i3s.up.pt/training?v=89

EMBO 3D Developmental Imaging Course

30 June - 08 July 2018 | IGC, Oeiras - Portugal

This EMBO Practical Course is aimed at junior researchers in developmental biology, struggling with imaging of cell movement and tissue morphogenesis in vivo using conventional microscopy techniques. Exploring techniques such as confocal, two-photon, light-sheet, and optical tomography with different samples is a major feature of the course. Several commercial partners contribute with state-of-the-art equipment for students to use during the course.

This year we will have:

• Six keynote lectures
• Four labs for eight different projects (selected from applications)
• One multi-photon microscope
• Three laser scanning and three spinning disk confocals
• Four light-sheet (commercial & custom built) and two optical tomography systems
• Seven workstations for 3D image analysis
• More equipments being confirmed...

The visualization, processing, analysis and publication of multidimensional images is also addressed, with both commercial and open source tools. Students are encouraged (in fact required!) to bring their own samples to test with multiple systems and to try other sample preparation and imaging protocols. The nine day course includes morning lectures with several renowned experts, and 6-8h of practicals/day with the support of over 20 teachers and more than ten high-end 3D imaging techniques. Students are encouraged to try new sample preparation protocols and as many imaging techniques as time permits. It also includes "micro" workshops on DIY building of microscopes and on image analysis. Although intense, this course is well appreciated for the breadth of techniques available and the inspiring and informal environment which favors discussions among peers.