Course on Optical Microscopy Imaging for Biosciences | 12th Edition
6-9 April 2021 | i3S, Porto
Imaging living cells are pivotal to understanding the biological processes. The course will present the state-of-art light microscopy techniques applied to study live cells and subcellular structures into different contexts (e.g., 2D and 3D culture systems, tissue explants, small model organisms, etc…). The program includes theoretical lectures headed by field specialists and live virtual practical modules which will offer the participants the opportunity to contact with cutting-edge microscopes (e.g., lattice light-sheet), learn how to manage live samples under the microscope and to get initiated into multidimensional image data analysis.
REGISTRATION
i3S members and PPBI nodes staff - 70€
External Participants - 100€
More information and registration: https://www.i3s.up.pt/training-detail.php?v=165
Introduction to digital bioimage analysis, 2nd Edition
September 23-25 and 28-30, 2020 | ONLINE COURSE
With the advent of the digital era, nowadays images are multidimensional numerical data that can be processed and analyzed quantitatively to extract more information. With this course, we intend to give a general introduction to ImageJ/Fiji (imagej.net/), show how to use it to extract quantitative data and how to write simple macros. This edition will be online (ZOOM) sessions with a practical approach.
CONTENTS
- Principles, techniques and ethics of digital bioimage processing.
- Basic commands of ImageJ/Fiji software.
- Working with multidimensional (2D, 3D, time-lapse, position) bioimages.
- Enhancement and processing of images.
- Extract quantitative numerical data.
- Design of simple workflows for automation of image analysis with ImageJ macros.
More information and registration: https://www.i3s.up.pt/training?v=119
Introduction to data analysis and image processing with MATLAB - 6th Edition
04-07 May 2020 | ONLINE COURSE
Quantification is an essential component in every scientific domain, and the ability to extract more information from the data is a fundamental asset. This is an introductory "hands-on" course where the concepts and techniques will be explored directly in the MATLAB environment. MATLAB is a well-established tool in scientific and technical computing, with key advantages such as being extremely versatile/powerful and easy to learn. This course is especially suited for people engaged in research wanting to start using MATLAB in the context of scientific data analysis and image processing (quantification). Participants are invited to bring their own data and an analysis problem of interest to be addressed in the course (as a project), with the help of the tutors.
- Previous experience with MATLAB is not required
PREREQUISITES
- Participants are asked to bring their laptops (a MATLAB trial version is available)
PROGRAM
Introduction to computer algorithms.
The MATLAB environment.
Variables and basic array operations.
Strategies for representing numerical/scientific problems in a computer.
Plotting data.
MATLAB programming.
Creating functions and scripts.
Automating data analysis.
Image analysis: images as arrays of data values; importing images and metadata; operations on image data; filtering; segmentation; morphological operations; properties measurements
More information and registration: https://www.i3s.up.pt/training?v=102
Course on Optical Microscopy Imaging for Biosciences | 12th Edition (Canceled)
23-27 March 2020 | i3S, Porto
The 12th edition of the Course on Optical Microscopy Imaging for Biosciences offers to the participants a comprehensive light microscopy course that explores the fundamentals of light microscopy and introduces the state-of-art of advanced fluorescence microscopy techniques. The course will flow from sample preparation to quantitative image analysis. It will include theoretical lectures taught by specialists in the field and hand-on practical modules supported by the major microscopy companies where the participants will have the opportunity to take work with high-end microscopy resources.
Program Topics
Specimen preparation;
Light proprieties and image formation;
Optical contrast techniques in transmission light microscopy;
Fluorescence microscopy techniques: wild-field, laser scanning confocal, two-photon, TIRF and light-sheet; Live cell imaging;
Analyzing biomolecular interactions and dynamics: FRET, FLIM, FRAP, PA, FC;
Super-resolution microscopy;
Quantitative image analysis.
REGISTRATION
Early Bird | Until February 7th, 2020
i3S members and PPBI nodes staff - 200€
External Participants - 270€
Payment deadline: February 13th, 2020
Late registration | From February 8th to February 29th, 2020
i3S members - 250€
External Participants - 325€
Payment deadline: March 5th, 2020
More information and registration: https://www.i3s.up.pt/training?v=116
February 29 - March 3, 2020 | Bordeaux, France
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