Introduction to digital bioimage analysis | 4th Edition

26-30 Setembro 2022 | Online

With the advent of the digital era, nowadays images are multidimensional numerical data that can be processed and analysed quantitatively to extract more information. With this course, we intend to give a general introduction to ImageJ/Fiji (imagej.net/), show how to use it to extract quantitative data. Also, the design of typical workflows to automate image analysis will be addressed.
Additionally, extra advanced topics will be available with scripting with macro language and image analysis with deep learning tools.
This course will be focused on the analysis of light microscopy images but is targeted at all people engaged in research.

CONTENTS

- Intro to Digital Image Analysis
- Workflow of image analysis
- Advanced ImageJ Plugins
- Batch processing: introduction to macro language
- Deep learning tools on image analyses

FEES:

More information and registration:  https://www.i3s.up.pt/training-detail.php?v=202

High Throughput Screening and Image Analysis for BioSciences, 6th edition

23-27 July 2022 | i3S

Fundamentally practical, this course aims to introduce the participants in experimental design, image acquisition, image and data analysis for high throughput (HT) experiments. These are essential skills when generating a massive amount of data, especially image-based data in the biosciences field. Lectures will be given by specialists in the field and participants will learn how to use state-of-the-art technology for HT experimentation and open-source software for image and data analysis. The course includes lectures and theoretical-practical classes plus Lab-Sessions (optional) where the attendants will get acquainted with the use of automated liquid handling equipment, plate readers and high content (HC) imagers. Interaction with HTS/HCS specialists will be fostered due to the restricted number of participants.

More information and registration:  https://www.i3s.up.pt/training-detail.php?v=201

Course on Optical Microscopy Imaging for Biosciences | 13th Edition

28 March -1 April 2022 | i3S, Porto

The 13th edition of the Course on Optical Microscopy Imaging for Biosciences offers to the participants a comprehensive light microscopy course that explores the fundamentals and introduces the state-of-art of advanced fluorescence microscopy techniques. The course will flow from sample preparation to quantitative image analysis. It will include theoretical lectures taught by specialists in the field and hands-on practical modules supported by the major microscopy companies where the participants will have the opportunity to take work with high-end microscopy technology.

More information and registration:   https://www.i3s.up.pt/training-detail.php?v=204

Batch analysis and macro development in ImageJ/Fiji: going beyond the basics | 3^rd Edition  

24-26 November 2021 | i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto

This course is designed for ImageJ/Fiji users who want to go beyond the basics and progress to more intermediate/advanced analysis with this software.

The course focuses on how to automate routine tasks (for conditions in which many images need to be processed), as well as how to deal with more complex image analysis situations and maximize information extraction.

Although ImageJ can be used with its graphical user interface, the implementation of a workflow to multiple images requires the development of macros and/or scripts for batch processing.

In this course, the process is tackled by building up from the development of very simple macros to more complex workflows, where images are processed and information acquired in an automated or semi-automated way.

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Biomolecular interaction analysis 2021: From molecules to cells 

25-29 October 2021 | Virtual 

A comprehensive and integrated characterization of molecular interactions is key to the understanding of life processes at a molecular level. Due to their complexity the study of binding events requires the gathering of data from multiple methods in order to build a complete picture of their function.

This EMBO Practical Course aims at a multidisciplinary approach to the study of molecular interactions from and in biological systems, integrating biochemical/biophysical methodologies with live cell microscopy techniques.

In this 5^th edition, this EMBO Practical Course will cover a range of methods used in the kinetics and thermodynamics characterization of binding events, such as:

Isothermal Titration Calorimetry (ITC),

Surface Plasmon Resonance (SPR),

Electrically Switchable nanolevers (switchSENSE), and

Microscale Thermophoresis (MST)

The bridge to in vivo will be crossed with the introduction of advanced light microscopy techniques, e.g.:

Fluorescence Resonance Energy Transfer (FRET) and

Fluorescence Lifetime Imaging (FLIM),

to study the dynamic interactions of proteins within the living cell.

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