PPBI comprises multiple light microscopy resources including high-end widefield microscopes, laser scanning and spinning disk confocal systems, two-photon microscopes and super-resolution.
PPBI
Portuguese Platform of BioImaging
PPBI - Portuguese Platform of BioImaging
National Research Infrastructure for Open-access to Bioimaging resources
Member of Portuguese Roadmap for Research Infrastructures of Strategic Interest
PPBI Euro-BioImaging Node
Light Microscopy
Widefield Fluorescence & Transmission Imaging
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- NBF @ INL*
- MICC @ CNC
- LiM @ iBiMED-UA
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF
- AFMF @ iBB-IST
- LMF @ UAlg
Mosaic Imaging
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- MICC @ CNC (Slide scanner)
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC (Slide scanner)
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF (Slide scanner)
- AFMF @ iBB-IST
- LMF @ UAlg
Laser Scanning and Spinning Disk Confocal
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- NBF @ INL*
- MICC @ CNC
- LiM @ iBiMED-UA
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF
- AFMF @ iBB-IST
- LMF @ UAlg
Multiphoton (2P)
Nodes:
- MICC @ CNC
- AIF @ IGC
- AFMF @ iBB-IST
Multidimensional in vivo timelapse experiments
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- MICC @ CNC
- LiM @ iBiMED-UA
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF
- AFMF @ iBB-IST
- LMF @ UAlg
High throughput microscopy
Nodes:
- BI @ i3S
- FCULMF @ FCUL
- AIF @ IGC
High-speed live cell microscopy
Nodes:
- ALM @ i3S
- AIF @ IGC
- BF @ iMM
Fluorescence Recovery After Photobleaching (FRAP)
Photoactivation (PA) and Photoconversion (PC)
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- MICC @ CNC
- LiM @ iBiMED-UA
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF
- AFMF @ iBB-IST
- LMF @ UAlg
Fluorescence Resonance Energy Transfer (FRET)
Nodes:
- ALM @ i3S
- BI @ i3S
- MF @ ICVS-UM
- MICC @ CNC
- LiM @ iBiMED-UA
- BF @ UC
- FMF @ CICS-UBI
- AIF @ IGC
- FCULMF @ FCUL
- BF @ iMM
- LMP @ CF
- AFMF @ iBB-IST
- LMF @ UAlg
Fluorescence-Lifetime Imaging Microscopy (FLIM)
Nodes:
- NBF @ INL*
- FCULMF @ FCUL
- AFMF @ iBB-IST
Fluorescence Correlation Spectroscopy (FCS)
Nodes:
- AFMF @ iBB-IST
Total Internal Reflection Fluorescence (TIRF)
Nodes:
- ALM @ i3S
- NBF @ INL*
- AIF @ IGC
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