Confocal Microscopes
Leica TCS-SP5 Multiphoton/Confocal Fluorescence Microscope equipped with:
- CW lasers: Ar ion laser (458, 465, 488, 496 and 514 nm) and HeNe (633 nm);
- Pulsed laser: Ti:Saphire (710-990 nm, 100 fs, 80 MHz);
- Microinjection: combination of InjectMan® 4 and Femtoject® 4i from Eppendorf;
- Autocorrelator and two APDs set for single-molecule FCS and FCCS;
- TCSPC module coupled to a DCC-100 PMT or 2 hybrid detectors.
Widefield Microscopes
Olympus IXplore IX83 Widefield Microscope equipped with:
- Digital Hamamatsu CMOS camera (C11440-36U);
- Imaging Workstation (HP Z2 SFF G4);
- cellSens dimension and standard software;
- Filter cubes: (1) Exciter filter: 340-390 nm, beam splitter: 410 nm, barrier filter: 420 nm; (2) Exciter filter: 470-495 nm, beam splitter: 505 nm, barrier filter: 510 nm; (3) Exciter filter: 530-550 nm, beam splitter: 570 nm, barrier filter: 575-625 nm.
- Objectives: UPLXAPO10X and UPLXAPO60XO.
Home-built confocal based microscope
Home-built confocal based microscope for single-molecule detection
