High content screening imaging system
IN Cell Analyzer 2000 (GE Healthcare) - Fully automated widefield fluorescence microscope equipped with:
- Objectives: 2x/0.1, 10x/0.45, 20x/0.45, 40x/0.95;
- Imaging from glass slides up to 1536-well plates;
- Fixed end-point assays or live-cell studies (temperature and CO2 control);
- Liquid handling (for Ca2+ flux assays, e.g.);
- Mosaic acquisition, 3D, deconvolution;
Others
Synergy 2 (BioTek) - High throughput multi-detection microplate reader. Examples of common applications are: absorbance measurements, visible wavelength fluorescence measurements and glow luminescence levels.
- Fluorescence Intensity
- Luminescence
- UV-visible Absorbance
- Temperature control
- Shaker
MultidropTM Combi Reagent Dispenser (Thermo-Scientific) - This bulk reagent dispenser quickly dispenses almost any type of liquids from reagents, to cells in suspension, to whole organisms. Can be operated inside safety cabinets. Examples of common applications are cells seeding, buffer exchanges, fixation, and washes.
- From 6- to 1536-well plates
- High speed and high precision
- From 0.5mL to 2500 mL
- From reagents to cells to whole organisms
JANUS with 4 Tip Arm and MDT Arm (PerkinElmer) - This automated liquid handling workstation allows for simple to complex pipetting workflows. Examples of common applications are compound transfer to assay plates, small volume liquid transfer, cherry picking.
- Automation of sample preparation
- Two pipetting arms: 4-tip VarispanTM and MDT
- Liquid transfers from any combination of laboratory containers including 384-well plates
- Compound transfer (100 nL pin-tool)
- Up to 24 microplates on the deck at one time (assay plates, tip boxes, reservoirs,...)
Liquidator 96 (Metler-Toledo) - Manual pipetting system. Allows pipetting all 96 wells at once. No need for programming, works as a normal multichannel pipette. Can be operated inside safety cabinets. Examples of common applications are: buffer or reagents transfer, 96 to 384 or reverse re-formatting, small volume transfer.
- Fast and easy pipetting like a manual pipette
- From 5 mL -200 mL
- Clean, Sterile, Filter Tips
- 96-384 well plates
ALPS 50 V-Manual Heat Sealer (Thermo-Scientific) - Microplate heat sealer. Examples of common applications are sealing plates for reagents incubation or for freezing.
- Secures tight seals around individual wells
- Sample protection of storage and reaction plates in applications including compound storage, sample archiving and PCR
- A wide range of hot seals, including pierceable, optically clear and permanent
Cell culture resources - The platform has independent cell culture equipment for projects being developed in the platform.
Image Analysis
Image Analysis - The scientific platform has powerful computer workstations to perform digital image analysis, Image/Data visualization and statistical analysis software. Examples of common applications are image processing, segmentation, and quantification.
Available software - CellProfilerTM, CellProfiler AnalystTM, IN Cell Investigator, ImageJ, shinyHTM, Spotfire®, GraphPad®
