PPBI

Leica TCS-SP8 - The Leica SP8 inverted confocal microscope is equipped with:

Laser lines: Diode 405, Ar (458, 476, 488, 496, 514), DPSS 561, HeNe 594 and HeNe 633 nm;
Objectives: 10x/ 0.40 dry, 20x/0.75 IMM/CORR, 40x/1.10 CORR water, 63x/1.40 oil and 63x/1.30 glycerol;
4 detectors: 2 Hybrid detector and 2 PMTs;
Adapters for 35 mm in diameter plates, well plates and 25 mm in diameter coverslips;
Tandem scanner (High-speed resonant plus conventional);
Motorized XY stage;
Environmental chamber with temperature and CO₂ control;
Allows: Lambda Scan; Mark & Find and Tile Scan (equipped with LasX "Navigator"); Timelapse; Dye Finder; FRAP; FRAP XT; FRET - AB (acceptor photobleaching); FRET - SE (stimulated emission); Live Data Mode; Photoconversion; Lightning; DIC;

Leica TCS-SP5 II - The Leica SP5 inverted confocal microscope is equipped with:

Laser lines: Diode 405, Ar (458, 476, 488, 496), DPSS 561, and HeNe 633 nm;
Objectives: 10x/ 0.40 dry, 20x/0.70 IMM/CORR, 40x/1.10 CORR water, 63x/1.40 oil and 63x/1.30 glycerol;
4 detectors: 1 Hybrid detector and 3 PMTs;
Adapters for 35 mm in diameter plates, well plates and 25 mm in diameter coverslips;
Tandem scanner (High-speed resonant plus conventional);
Motorized XY stage;
Environmental chamber with temperature and CO₂ control;
Allows: Lambda Scan; Mark & Find and Tile Scan; Timelapse; Dye Finder; FRAP; FRAP2; FRET - AB (acceptor photobleaching); FRET - SE (stimulated emission); Live Data Mode; Photoconversion; DIC;

Andor Revolution XD (Andor Technology) - Inverted Spinning disk confocal microscoped equipped with:

Objectives: 10x/0.40 air, 20x/0.70 air, 40x/1.30 oil, 60x/1.42 oil, 100x/1.40 oil;
Lasers: 488nm (50mW) and 561nm (50mW);
Filters on the confocal system: Semrock dichroic dual mirror 488/561; 488 dichroic mirror; Semrock dual 488/561 emission filter; 488 emission filter;
iXon^EM+ DU-897 with 2x port coupler camera;
X-Y Motorized stage;
Fast Z stage;
Allows: Motorized operation; Automatic multi-dimensional image acquisition; Environmental chamber with temperature and CO2 control (big incubator - allows multi-well plates, slide chambers and 35mm dishes), DIC;

Zeiss Axio Imager Z1 - Upright widefield microscope equipped with:

Fluorescence filters for: 365, 436, 470, 500, 550, and 640 nm;
Objectives: 5x air, 10x/0.30 Ph1 air, 20x/0.50 Ph2 air, 40x/1.30 Ph3 oil, 63x/1.40 oil DIC, and 100x/1.40 oil DIC;
Allows: Motorized operation; Phase contrast (10-40x) and DIC (63-100x); Automatic acquisition of multi-channel images and Z planes;

Zeiss Axio Imager Z1 Apotome - Upright widefield microscope equipped with:

Fluorescence filters for: 365, 450, 470, 550, and 572 nm;
Objectives: 10x/0.30 air, 20x/0.8 air, 40x/1.30 oil, 63x/1.40 oil DIC, and 100x/1.40 oil DIC;
Allows: Motorized operation; Optical sectioning with Apotome; Automatic acquisition of multi-channel images and Z planes;

Zeiss Axiovert 200M - Inverted widefield microscope equipped with:

Fluorescence filters for: 387, 472, 562, and 628 nm;
Objectives: 20x/0.3 Ph1 air, 40x/1.40 oil DIC, 40x/1.30 Ph3 oil, 63x/1.40 oil DIC;
CoolSnap HQ (Roper) camera;
X-Y Motorized stage;
NanoPiezzo Z stage;
Anti-vibration passive table;
Allows: Motorized operation; Automatic multi-dimensional image aquisition; Environmental chamber with temperature and CO₂ control;

Leica DMI6000 - Inverted widefield microscope equipped with:

Objectives: 10x/0.30 Ph1 air, 20x/0.40 CORR Ph1 air, 40x/0.60 CORR Ph2, 63x/1.30 glycerol;
Fluorescence filters for: 340, 460, 540, and 590 nm & CFP/YFP/GFP/MRFP External filter sets;
Hamamatsu FLASH4.0 camera;
X-Y Motorized stage;
Allows: Motorized operation; Automatic multi-dimensional image acquisition; Environmental chamber with temperature and CO2 and O2 (hypoxia) control (small incubator - allows ibidi slide chambers and 35mm dishes);

Leica DMI6000 - Inverted widefield microscope equipped with:

Objectives: 10x/0.30 Ph1 air, 20x/0.40 CORR Ph1 air, and 40x/0.60 CORR Ph2;
Fluorescence filters for: 340, 460, 540, and ;
Hamamatsu FLASH4.0 camera;
X-Y Motorized stage;
Allows: Motorized operation; Automatic multi-dimensional image acquisition; Environmental chamber with temperature and CO2 control (big incubator - allows multi-well plates, slide chambers and 35mm dishes);

Nikon Ti TIRF - Inverted motorized microscope equipped with:

Objectives: 10x/0.45 WD 4mm, 40x/0.95 WD 0.24mm, 60x/1.4 oil WD 0.13mm, and 100x/1.47 oil WD 0.12mm;
Multiple combinations with Lumencor SpectraX light engine and external emission filter wheel;
Andor iXon888;
Allows: Full Motorized operation; Automatic acquisition of multi-channel images, Z planes, and positions; Environmental chamber with temperature and CO₂ control (big incubator - allows multi-well plates, slide chambers and 35mm dishes); DIC; TIRF. Possibility to implement super-resolution applications.

Descartes – Image processing and analysis workstation equipped with:

PPBI - Portuguese Platform of BioImaging