PPBI

Portuguese Platform of BioImaging

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PPBI

Portuguese Platform of BioImaging

Home
About
Portfolio
Nodes
Training
News&Events
Foruns
Jobs
Contact Us

PPBI - Portuguese Platform of BioImaging

National Research Infrastructure for Open-access to Bioimaging resources

Member of Portuguese Roadmap for Research Infrastructures of Strategic Interest

PPBI Euro-BioImaging Node

The new Portuguese Platform of BioImaging (PPBI) Node is a multi-sited, multimodal Node covering biological imaging.

PPBI represents the national biological microscopy imaging community of Portugal.

PPBI services focus on advanced microscopy and image data analysis for a wide range of life science domains - from cell and developmental biology, to neurosciences, oncobiology, immunology, infection, and regenerative medicine.

Tissue Clearing Online Workshop

18-20 May 2021 | ONLINE

Tissue clearing is a technique that enables visualization of opaque organs and tissues in 3-dimensions (3-D) by turning tissue transparent. The major challenge that underlies most of the unsuccessful applications of tissue clearing is that every application requires its own protocol and optimization approach.

This 3 days online workshop will cover various topics in tissue clearing, from protocol optimization, light-sheet microscopy imaging to 3D visualization and quantification. This is an opportunity for participants to learn and discuss tissue clearing protocols and applications and to contact with state-of-the-art light microscopy systems for imaging of the cleared tissue samples, guided by an experienced staff. Participants will also explore processing and image analysis workflows to extract the best information from the data.

BioImage Analysis for High Content Screening

25 - 28 May 2021 | ONLINE

High Content Screening generates a massive amount of image-based data in the biosciences field. Images contain diverse and valuable quantifiable data, however, many researchers lack the knowledge to extract those data in a practical, fast, and repetitive way.
Fundamentally practical, in this modular course, participants will get acquainted with several open-source image analysis software (ImageJ/Fiji, ilastik, CellProfiler, CellProfiler Analyst, Cytoscape and IDEAS®) designed to deal with large amounts of images. Experimental data will be used as datasets for image segmentation and quantification.
This course is organized in modules. Therefore, participants are free to choose the image analysis tools more convenient to their own work.

Introduction to digital bioimage analysis, 3rd Edition

12-15 October 2021| i3S

With the advent of the digital era, nowadays images are multidimensional numerical data that can be processed and analyzed quantitatively to extract more information.
With this course, we intend to give a general introduction to ImageJ/Fiji (imagej.net/), show how to use it to extract quantitative data, and how to write simple macros

Batch analysis and macro development in ImageJ/Fiji: going beyond the basics

24-26 November 2021 | i3S

This course is designed for ImageJ/Fiji users who want to go beyond the basics and progress to more intermediate/advanced analysis with this software. The course focuses on how to automate routine tasks (for conditions in which many images need to be processed), as well as how to deal with more complex image analysis situations and maximize information extraction. Although ImageJ can be used with its graphical user interface, the implementation of a workflow to multiple images requires the development of macros and/or scripts for batch processing. In this course, the process is tackled by building up from the development of very simple macros to more complex workflows, where images are processed and information acquired in an automated or semi-automated way.

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Ask for collaborations!

PPBI

Get to know the Huygens Remote Manager!

PPBI

PPBI Nodes' leaders have met at IGC!

PPBI

NEUBIAS

PPBI

Portfolio

Ask for collaborations!

PPBI

Get to know the Huygens Remote Manager!

PPBI

PPBI Nodes' leaders have met at IGC!

PPBI

NEUBIAS

PPBI

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Events

ELMI 2021

22-25 June 2021 | Online Meeting

The annual Meeting of the European Light Microscopy Initiative (ELMI) is an internationally recognized meeting on light microscopy and a unique blend of lectures and hands-on workshops on the newest microscopy research and techniques aimed at a life sciences audience.

mmc2021 - Microscience Microscopy Congress 2021

5 - 9 July 2021 | ONLINE

mmc2021 will encompass:
- an international scientific virtual conference (6 - 8 July) of six parallel streams, covering an enormous range of microscopy, cytometry and imaging topics
- a huge FREE to ATTEND virtual exhibition (6 - 8 July) with companies both large and small showcasing their latest equipment and available to answer your questions
- virtual meetings & workshops covering the latest emerging topics (5 - 9 July)

CTLS2021

13-15 September 2021 | ONLINE

Core Facilities are key-players in modern institutions. Their still emergent role inevitably entails new paradigms regarding their scope and structure.

The CTLS2021 conference aims to address several of these points, contributing to shape the future of the technical and scientific landscape of life sciences in Europe.

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Gallery

Confocal Microscopy

Human-induced Pluripotent Stem Cell-Derived cardiomyocyte
https://doi.org/10.1016/j.bbrc.2018.03.198
IBB, IST

Ratiometric FRET

Ana Seixas - IBMC/i3S

Confocal microscopy

Mice adipose tissue infected with Trypanosoma brucei
Mariana De Niz, iMM

CH-STED microscopy

Coherent-hybrid stimulated emission depletion microscopy
António Pereira - IBMC/i3S

High content screening

i3s

Confocal microscopy

Light-sheet microscopy

Zebrafish
VDR group, IBMC/i3S

CLEM

Correlative light and electron microscopy (CLEM)
Sónia Gomes Pereira
[work developed in the laboratories of Jörg D. Becker and Mónica Bettencourt-Dias (IGC)]
https://doi.org/10.1101/2020.12.21.423647

Micro-CT

Mouse Trabeculae
INEB/i3S

Confocal microscopy

Schwann cells
Ana Seixas - IBMC/i3S

Live Cell Imaging

Sónia Henriques IBMC, i3S

Confocal Microscopy

In vitro infection of G. mellonella hemocytes with S. Typhimurium strains
doi: 10.1128/AEM.02850-17
IBB, IST

FLIM

Fluorescence lifetime imaging microscopy
https://doi.org/10.1021/jp310396v
IBB, IST

Confocal Microscopy

Joana Pereira - IBMC, i3S

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PPBI

PPBI

PPBI - Portuguese Platform of BioImaging

PPBI Euro-BioImaging Node

Highlights

PPBI is a new node of Euro-BioImaging!

Tissue Clearing Online Workshop

BioImage Analysis for High Content Screening

Introduction to digital bioimage analysis, 3rd Edition

Batch analysis and macro development in ImageJ/Fiji: going beyond the basics

Ask for collaborations!

Get to know the Huygens Remote Manager!

PPBI Nodes' leaders have met at IGC!

NEUBIAS

Events

ELMI 2021

mmc2021 - Microscience Microscopy Congress 2021

CTLS2021

Gallery

Confocal Microscopy

Ratiometric FRET

Confocal microscopy

CH-STED microscopy

High content screening

Confocal microscopy

Light-sheet microscopy

CLEM

Micro-CT

Confocal microscopy

Live Cell Imaging

Confocal Microscopy

FLIM

Confocal Microscopy

PPBI - Portuguese Platform of BioImaging

PPBI Euro-BioImaging Node

Highlights

Training

Events

Gallery

Confocal Microscopy

Ratiometric FRET

Confocal microscopy

CH-STED microscopy

High content screening

Confocal microscopy

Light-sheet microscopy

CLEM

Micro-CT

Confocal microscopy

Live Cell Imaging

Confocal Microscopy

FLIM

Confocal Microscopy