PPBI comprises multiple light microscopy resources including high-end widefield microscopes, laser scanning and spinning disk confocal systems, two-photon microscopes and super-resolution.

 

TECHNIQUES AVAILABLE

 NODES

Multichannel (widefield) fluorescence imaging + transmission imaging
CBC/UA, CICS-UBI, CNC, CF, FCUL, IBMC, ICVS/3B-UM, IGC, IMM, INEB, IST, UC, UAIg
3D imaging and reconstruction with laser scanning or spining disk confocals, but may also have multiphoton (2P) or light-sheet (LSM).
IBMC, INEBICVS/3B-UM
CBC/UACICS-UBI, CNC(2P!)UC
IGC (+2P, + LSM + EM tomography), CF (+LSM), IMM (+LSM), FCUL, IST(+2P)
UAIg
Multidimensional in vivo timelapse experiments (ie, equipped with incubators and environmental control)
CBC/UACICS-UBICNCCFFCULIBMCICVS/3B-UMIGCIMMINEBISTUCUAIg
Mosaic imaging  (imaging of large areas with multiple fields and stitching of adjacent fields; eg large tissue sections or whole embryos/organs)
IBMC, ICVS/3B-UM, INEB,  
CICS-UBICNC,UC
CF (+slide scanner) IGC (+slide scanner),  MM (+slide scanner), IST
UAIg
High throughput microscopy; typically fully automated scope with protocols for acquiring automatically multiple fields in multi-well plates or dishes.
IGC; FCULIBMC
Fluorescence Recovery After Photobleaching (FRAP),
Photoactivation (PA) and Photoconversion (PC)
CBC/UA, CICS-UBI, CNC, CF, FCUL, IBMC, ICVS/3B-UM, IGC, IMM, INEB, IST, UC, UAIg
Fluorescence Resonance Energy Transfer (FRET)
CBC/UA, CICS-UBI, CNC, CF, FCUL, IBMC, ICVS/3B-UM, IGC, IMM, INEB, IST, UC, UAIg
Fluorescence-Lifetime Imaging Microscopy (FLIM) and Fluorescence Correlation Spectroscopy (FCS)
IST
High-speed live cell microscopy and analysis (typicaly acquisition of multiple images /second)
IBMCIGCIMM
"Super Resolution" and Single Mol. Localization techniques
IBMC (STED)
IGC
 (STORM; TIRF, SRRF, SIM); ITQB (SIM)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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