The MICC-CNC offers a wide range of different instruments for your research, from epifluorescence microscopy, to high-speed confocal microscopy and laser catapulting microdissection. The systems are prepared for advanced applications, including live cell imaging, and single-cell calcium measurement, enabling researchers to image dynamic events and molecular interactions. The P.A.L.M. laser microdissection microscope is a perfect tool for the isolation of the different cell population within a sample, allowing its full characterization.
LSM 710 – 34Ch (Axio Observer) - Inverted microscope with a motorized stage equipped with:
LSM 710 – 34Ch -T-PMT (Axio Observer) - Inverted microscope with DIC and motorized stage equipped with:
Cell Observer SD Spinning-Disk Microscope - High speed fully motorized confocal microscope equipped with:
Axio Observer Z1 Live Cell Imaging Station - Fully motorized inverted microscope suitable for live cell imaging experiments, equipped with:
Axio Imager Z2 Up-right with ApoTome2 and Stereo Investigator software - Fully motorized upright microscope equipped with:
Axiovert 200 Single Cell Calcium Imaging System - Inverted microscope for single cell calcium measurements:
Axioskop 2 plus Widefield microscope - Upright widefield fluorescence microscope equipped with:
PALM MicrobeamRel 4.2 Laser Microdissection microscope - Inverted Microscope Axio Observer Z1 equipped for:
PALM Robot Microbeam Laser Microdissection microscope - Inverted Microscope Axiovert 200M equipped for:
The AIF offers you can a wide range of different instruments for your research. From the convencional widefield and confocal microscopes to the super-resolution microscopes, you can select the best equipment for your experiments.
Leica SP5 Inverted - The Leica SP5 confocal microscope is equipped with:
Leica SP5 Live Upright (HyD) - Another SP5, with a few notable differences:
Andor Spinning Disk - Spinning disk confocal microscopy:
Andor Spinning Disk W1 - This confocal microscope allows acquisition of images at very high frame rates with minimum illumination of samples in a broader field of view when compared with other spinning disks.
Multi-Photon Microscope - A multi-photon, or two-photon, microscope uses instrumentation similar to that of a laser scanning confocal microscope: A laser source for sample excitation, a scanhead with galvanometer controlled mirrors (or acousto-optic deflectors) to scan the excitation beam, and photomultiplier tubes to detect fluorescent signals. However, the confocal image differs in that optical sectioning is obtained using excitation in multi-photon microscopy whereas in confocal microscopy it is achieved using the emission pinhole.
Deltavision OMX (SIM) - The Deltavision OMX is a structured illumination super-resolution microscope that allows the visualization of structures or objects below the theoretical limit of resolution. This microscope technique uses the structured illumination approach which is simple to use and gives fast results with minimal to medium processing, allowing users to obtain super-resolution images with ease. The resolution limit of this technique is about half the theoretical limit of conventional microscopes at approximately 100nm.
DSTORM (SMLM) - The dSTORM is another super-resolution microscope based on single-molecule localization microscopy technique, more specifically, Stochastic Optical Reconstruction Microscopy. This technique relies on acquiring a big number of frames over time to distinguish different molecules while still pinpointing their location with high accuracy. The temporal distinction is achieved by inducing a "dark" triple state of energy which will not emit fluorescent light, allowing a distinction between different fluorophores in very small spaces, even below the actual resolution limit. This technique is easy to use but the sample preparation can be tricky and it requires big data storage and high processing power and expertise to properly reconstruct reliable and useful data.
Lightsheet Microscope - Light Sheet Microscopy (LSM) is a fluorescence microscopy technique, where the illumination is done perpendicularly to the detection. The technique shapes the illumination laser beam into a rectangle and then focuses it down only in one direction, using a cylindrical lens (SPIM) or galvanometric mirrors (DSLM). This forms a thin "sheet of light" right in the focal plane of the detection objective, illuminating the whole sample plane at the same time. A CMOS camera records the fluorescent signal. This allows obtaining images of a big area in a fast way with good sectioning of the sample and out-of-focus light suppression. LSM is especially well suited for the investigation of the development of large samples to study features (such as gene expression patterns) that require high resolution while being extended over a large volume and a long period of time. It has been successfully used to track developmental processes on Zebrafish, Drosophila fly, C. elegans nematodes or Arabidopsis plants among others.
DeltaVision - The DeltaVision is a fully automated research microscope that offers:
Leica DMRA2 - The Leica DMRA2 is a fully automated research microscope that offers high sensitivity to fluorescence and interference contrast (DIC). The powerful image-processing Leica DMRA2 software operates the microscope with precision.
Leica DMLB2 - The Leica DMLB2 is an upright microscope for experiments with Brightfield and DIC.
Nikon HCScreening - The Nikon HCS is an inverted widefield microscope with complete automation possibility. This microscope allows users to acquire large amounts of data with ease by giving instructions to the software at the beginning and it will do the rest. It is equipped with:
Leica and Zeiss HCScreening - The Leica and Zeiss HCS are inverted widefield microscopes with an automated stage and fast sets of filter wheels that allow automatic image acquisition (screening) and large field tile/grid stitching through a fast acquisition protocol. These microscopes are prepared for automatically acquiring images at a lower magnification, where it will identify an object or structure of interest, and then acquire them at a higher and better resolution magnification.
Zeiss Stereo Lumar - Zeiss' Stereo Lumar is a fluorescence capable and fully automated stereoscope with an increased focusing range, letting you take pictures of tissues of whole adult fish, mice, and even larger animals. With it's high resolution, high-quality ApoLumar 1.2 lens, variable zoom, and large working distance, users have the flexibility to choose between their desired zoom without sacrificing quality.
Hamamatsu Aequoria - The Aequoria MDS system is a macroscopic system to acquire luminescence in large samples like living large organisms, bacterial plates, plants or well plates, making it suitable for high-throughput screens. With an EMCCD chip, it can detect the smallest signals on your samples with ease.
OPenT - The OPenT microscope is an Optical Projection Tomography system which allows seeing big samples such as whole organs or embryos without having to open them or taking the organ out. Samples need to be clear and transparent. This system works basically as a CT scan but uses light instead of x-rays for the image acquisition. For more details, advantages, and limitations, please speak with the Advanced Imaging personnel.
The Bioimaging Facility offers you a wide range of different instruments for your research.
3i Marianas SDC – spinning disk confocal microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=3i_marianas_sdc).
Zeiss Cell Observer SD – spinning disk confocal microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_cell_observer_sd).
Zeiss LSM 880 with Airyscan – confocal laser point-scanning microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_880).
Zeiss LSM 710 – confocal laser point-scanning microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lsm_710).
Leica SP8 MP – multiphoton microscope (with Insight DS+ laser for multiphoton and 488 nm Ar laser for confocal imaging, upright for intravital imaging)
Zeiss Lightsheet Z1 – lightsheet fluorescence microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lightsheet_z1).
Zeiss Cell Observer – motorized widefield fluorescence microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_cell_observer).
Zeiss Axiovert 200M – motorized widefield fluorescence microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_axiovert_200m).
Leica DM5000B – widefield fluorescence microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=leica_dm5000b).
Zeiss AxioZoom V16 – fluorescence stereomicroscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_axiozoom_v16).
Zeiss Lumar V12 – fluorescence stereomicroscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=zeiss_lumar_v12).
Leica DM2500 – brightfield microscope (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=leica_dm2500).
IVIS Lumina – fluorescence/bioluminescence imaging system (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=ivis_lumina).
Big Guy – Image processing and analysis workstation (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=big_guy).
Colossus – Image processing and analysis workstation (https://imm.medicina.ulisboa.pt/facility/bioimaging/doku.php?id=colossus).
Leica SP5 Inverted - The Leica SP5 confocal microscope is equipped with:
The FCULMF offers you a wide range of different instruments for your research.
Leica SP8 Confocal - Fully motorized confocal equipped with:
Olympus BX51 widefield color - Manual widefield microscope equipped with:
Olympus BX60 widefield B&W - Manual widefield microscope equipped with:
JEOL SEM -
Zeiss Lumar V.12 stereoscope color - Fully motorized stereoscope equipped with:
Leica Cryostat - Semi-automated precision microtome for frozen sections.
Liquidator 96 pipette - Manual 96 channel pipette from Mettler Toledo, compatible w/ 96 and 384 well plates.
Tecan Hydrospeed plate washer - Automated plate washer for cells, beads and ELISAs in 96- and 384-well format.
Voxel2 analysis workstation; Voxel3 analysis workstation; Voxel4 analysis workstation - Intel Core i7-4790 CPU @ 3.60GHz; 16 GB RAM; 256 GB SSD + 1TB HDD; AMD Radeon HD 5450 1GB RAM; 22'' @ 1920 x 1080 x 60 hertz.
Voxel5 3D analysis workstation - Intel Core i7-7700 CPU @ 4.20GHz; 32 GB RAM; 120 GB SSD + 240 GB SSD + 2TB HDD; NVidia GTX 1060 6GB RAM; 24'' @ 1920 x 1080 x 60 hertz + 19'' @ 1280 x 1024 x 60 hertz; Bitplane Imaris 9.2.x.
Voxel6 3D analysis workstation - Intel Core i5-2500 CPU @ 3.30GHz; 16 GB RAM; 120 GB SSD + 1TB HDD; NVidia GTX 1050Ti 4GB RAM; 24'' @ 1920 x 1080 x 60 hertz; Visage Imaging Amira 5.3.3.
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