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Equipment @ MICC-CNC

 

The MICC-CNC offers a wide range of different instruments for your research, from epifluorescence microscopy, to high-speed confocal microscopy and laser catapulting microdissection. The systems are prepared for advanced applications, including live cell imaging, and single-cell calcium measurement, enabling researchers to image dynamic events and molecular interactions. The P.A.L.M. laser microdissection microscope is a perfect tool for the isolation of the different cell population within a sample, allowing its full characterization.

 

 

The MICC-CNC offers a wide range of different instruments for your research, from epifluorescence microscopy, to high-speed confocal microscopy and laser catapulting microdissection. The systems are prepared for advanced applications, including live cell imaging, and single-cell calcium measurement, enabling researchers to image dynamic events and molecular interactions. The P.A.L.M. laser microdissection microscope is a perfect tool for the isolation of the different cell population within a sample, allowing its full characterization.

 

Confocal Microscopes

 

 

LSM 710 – 34Ch (Axio Observer) - Inverted microscope with a motorized stage equipped with:

  • Laser lines: Diode 405nm, AR multiline (458nm, 488nm, 514nm), 561nm and 633nm, plus  a QUASAR detection unit (34 channels);
  • The system is prepared for live cell imaging assays: temperature and CO2 control (small incubator);
  • Objectives: Plan-Apochromat 20x/0,8; Plan-Apochromat 40x/1,4 oil; Plan-Apochromat 63x/1,4 oil;
  • Software: Zen Black 2012 - ZEN Module Measurement /ZEN Module Multi-Channel / ZEN Module Image Analysis / ZEN Module Time Lapse / ZEN Module Z Stack / ZEN Module Extended Focus / ZEN Module Autofocus / ZEN Module Colocalisation / ZEN Module Spectral Unmixing and ZEN Module Physiology.

 

 

LSM 710 – 34Ch -T-PMT (Axio Observer) - Inverted microscope with DIC and motorized stage equipped with:

  • Laser lines: Diode 405nm, AR multiline (458nm, 488nm, 514nm), 561nm and 633nm, plus a QUASAR detection unit (34 channels); T-PMT and BIG detector (filter set4-green-red 500-550/570-610);
  • The system is prepared for live cell imaging assays: temperature and O2/CO2 control (XL incubator);
  • Objectives: Plan-Apochromat 20x/0,8; Plan-Apochromat 40x/1,4 oil; LCI Plan-Neofluar 63x/1,3Imm Corr ;Alpha Plan-Apochromat 100x/1,46 oil DIC;
  • Software: Zen Black 2012 - ZEN Module Measurement /ZEN Module Multi-Channel / ZEN Module Image Analysis / ZEN Module Time Lapse / ZEN Module Z Stack / ZEN Module Extended Focus/ ZEN Module Autofocus / ZEN Module Colocalisation / ZEN Module Spectral Unmixing and ZEN Module Physiology.

 

 

Cell Observer SD Spinning-Disk Microscope - High speed fully motorized confocal microscope equipped with:

  • Yokogawa CSU-X1M5000 disk;
  • 3 solid state lasers (405nm-50mW, 488nm-100mW, and 561nm-50mW);
  • EM-CCD high sensitivity camera (Evolve Delta);
  • Direct FRAP module, allowing live imaging and photomanipulation assays;
  • Definite Focus and DIC;
  • Microscope with ASI motorized stage with top plate Z-piezo;
  • The system is equipped with a large incubator and small stage incubator for temperature, CO2 and humidity control;
  • Objectives: Plan-Apochromat 20x/0,8; LCI-Planneofluar 63x/1,3 corr DIC water/glycerol; Alpha Plan-ApoChromat 100x/1,46 oil DIC;
  • Software: Zen System- Blue 2012 with ZEN Module Measurement / ZEN Module Multi-Channel / ZEN Module Image Analysis / ZEN Module Time Lapse / ZEN Module Z Stack / ZEN Module Extended Focus / ZEN Module Autofocus / ZEN Module Colocalisation /ZEN Module Physiology / ZEN module Tiles and Positions.

     

     

Widefield Microscopes

 

 

Axio Observer Z1 Live Cell Imaging Station - Fully motorized inverted microscope suitable for live cell imaging experiments, equipped with:

  • A large size incubator for temperature control and a small stage incubator for temperature control and CO2 supply;
  • The system is equipped with two monochromatic cameras: a CCD camera (AxioCam HRm) and a CMOS camera (Hamamatsu Orca Flash4.0);
  • Objectives: EC-PlanNeofluar 10x/0,3; Plan-Apochromat 20x/0,8; Plan-Apochromat 40x/0,95 Korr; EC-PlanNeofluar 40x/1,3 oil; Plan-ApoChromat 63x/1,4 oil;
  • Software: Zen Pro Blue 2012 with ZEN Module Measurement / ZEN Module Multi-Channel ZEN Module Z Stack / ZEN Module Time series / ZEN Module Tiles and Position/ZEN Module Autofocus / ZEN Module Experiment Designer.

 

 

Axio Imager Z2 Up-right with ApoTome2 and Stereo Investigator software - Fully motorized upright microscope equipped with:

  • Apotome2 (for structural illumination);
  • Stereo Investigator software (for stereology studies);
  • Two high-resolution cameras (AxioCam HRm and AxioCam HRc);
  • Motorized DIC for the 63x objective;
  • Objectives: EC-PlanNeofluar 5x/0,16; Plan-ApoChromat 20x/0,8; Plan-ApoChromat 40x/1,3 oil; Plan-ApoChromat 63x/1,4 oil;
  • Software: Zen Pro Blue 2012 with ZEN Module Measurement / ZEN Module Multi-Channel ZEN Module Z Stack / ZEN Module Time series / ZEN Module Tiles and Position/ZEN Module Autofocus / ZEN Module Experiment Designer/ZEN Module Apotome.

 

 

Axiovert 200 Single Cell Calcium Imaging System - Inverted microscope for single cell calcium measurements:

  • Coupled with a Lambda DG-4 and a perfusion system;
  • Highly sensitive camera (CoolSnap HQ) - allowing to follow fast changes in ion concentrations in dual wavelength ratio imaging applications;
  • Objectives: PlanNeofluar 5x/0,15; PlanNeofluar 20x/0,5; Fluar 40x/1,3 oil; Fluar 100x/1,3 oil;
  • Software: Metafluar (Molecular Devices).

 

 

Axioskop 2 plus Widefield microscope - Upright widefield fluorescence microscope equipped with:

  • Phase contrast objectives;
  • A color CCD camera for brightfield/fluorescence imaging (AxioCam HRc);
  • Objectives: PlanNeofluar 1,5x/0,005; PlanNeofluar 5x/0,15; PlanNeofluar 20x/0,5; PlanNeofluar 40x/0,75; PlanNeofluar 63x/1,25 oil; PlanNeofluar 100x/1,3 oil;
  • Software: Axiovision with Multichannel Module.

 

 

Others

 

 

PALM MicrobeamRel 4.2 Laser Microdissection microscope - Inverted Microscope Axio Observer Z1 equipped for:

  • Laser Microdissection using UV laser and catapulting technology and with CapMover II;
  • Objectives: Fluar 5x/0,25; LD PlanNeofluar 40x0,6 corr ; LD PlanNeofluar 63x0,75 corr;

 

 

PALM Robot Microbeam Laser Microdissection microscope - Inverted Microscope Axiovert 200M equipped for:

  • Laser Microdissection using UV laser and catapulting technology;
  • Objectives: Fluar 5x/0,25; LD-PlanNeofluar 20x/0,4; LD-PlanNeofluar 40x/0,6; Plan-ApoChromat 63x/1,4 oil;
  • Software: Axiovision with Multichannel / z-stack, MosaicX, Timelapse / Extended Focus / Automeasure Plus / and Commander modules;
  • PALM Robot Software with Autofocus, LMPC and Multichannel Fluorescence Modules.