The ABBE offers you a wide range of different instruments for your research. From the conventional widefield and confocal microscopes to the super-resolution microscopes, you can select the best equipment for your experiments.
The ABBE offers you a wide range of different instruments for your research. From the conventional widefield and confocal microscopes to the super-resolution microscopes, you can select the best equipment for your experiments.
Confocal Microscopes
Zeiss LSM 880, Laser scanning confocal microscope - The Zeiss LSM 880 is an inverted confocal laser-scanning microscope that allows you to optically section fluorescent samples for high-contrast images of thick specimens at high resolution. The laser scanning unit contains a filter-free spectral separation module allowing you to specify any wavelength detection range between 400-700 nm freely. For excitation, the system is equipped with lasers from violet to far-red:
- 405, 458, 488, 514, 561 and 633 nm.
In addition to a T-PMT detector for brightfield acquisitions, the system is equipped with:
- two standard PMT detectors and a QUASAR spectral GaAsP detector that can be further divided into up to eight sub-detectors.
Also, the LSM 880 has AiryScan capability for extended resolution applications. This technology makes it possible to reach lateral and axial resolutions of 140 nm and 400 nm respectively by creating a 0.2AU virtual pinhole for maximum resolution while maintaining a light collection efficiency of a 1.25AU pinhole.
Lightsheet Microscope
Zeiss Lightsheet Z.1, Lightsheet microscope - The Zeiss Lightsheet Z.1 is a light sheet fluorescence microscope able to image optical sections of large samples at a sub-cellular resolution and very fast rates, with very little excitation power. Equipped with:
- A high-speed and highly sensitive sCMOS camera (dual PCOEdge cameras with up to 30 fps speed).
Widefield Microscopes
Zeiss AxioImager M2, Widefield fluorescence scanning microscope - The Zeiss AxioImager M2 is a fully motorized upright widefield microscope suitable for semiautomated fluorescence and brightfield imaging. The system is equipped with:
- A high-efficiency fluorescence filter sets and an HXP 120V metal-halide lamp for fluorescence excitation;
- A monochrome CCD camera (Zeiss AxioCam MRm);
Zeiss AxioScan.Z1, Automated widefield slide scanner - The Zeiss AxioScan.Z1 is a fully automated brightfield and fluorescence slide scanner for high volume widefield imaging. This system is capable of scanning up to 100 whole slides in a single experiment run, and it can reconstruct “virtual slides” at high-resolution by stitching tiled scans. The system is equipped with:
- Fluorescence filters for a wide range of dyes;
- An option of Colibri.2 LED illumination or the metal halide HXP fluorescent lamp;
- A Hitachi 3 CCD color camera;
- A high-sensitivity monochromatic sCMOS camera (Hamamatsu Orca Flash 4.0 v2).
Olympus BX61 (Stereo Investigator), Widefield fluorescence microscope - The Olympus BX61 is an upright widefield microscope suitable for UV/blue/green fluorescence and brightfield imaging. It is equipped with:
- A motorized stage for tracing and analyzing structures beyond a single field of view;
- The MBF Bioscience software packages;
- The Stereo Investigator 9.13;
- Neurolucida 9.13 software.
Zeiss AxioScope A1 (DIC), Brightfield microscope - The Zeiss AxioScope A1 is an upright brightfield microscope with:
- A HAL 100 lamp for transillumination;
- A DIC unit.
Zeiss AxioScope A1 (DIC), Brightfield microscope - The Zeiss AxioScope A1 is an upright brightfield microscope with a HAL 100 lamp for transillumination and a DIC unit for high-contrast brightfield imaging using Differential Interference Contrast (DIC).
With this system, you can image both living samples in aqueous media or fixed samples where tissue clearing has been performed. Samples are mounted not on coverslips but inside special chambers that additionally provide fine temperature control to maintain organism health during live time-lapse experiments.
Zeiss Lumar V12, Fluorescence stereo microscope - The Zeiss Lumar.V12 is a fluorescence stereomicroscope suitable for the 3D analysis of large specimens in fluorescent and brightfield light. The system is equipped with:
- A cold KL2500 LCD light-source well-suited for dissections;
- An HXP 120C lamp;
- High-efficiency filter sets for multi-channel fluorescence observations;
- Single and time-lapse image capture is possible on this microscope using a sensitive cooled CCD camera, and the motorized Z-drive enables three-dimensional acquisitions.
Zeiss Discovery V8, Fluorescence stereo microscope (fly dissection) - The Zeiss Discovery.V8 is a fluorescence stereomicroscope suitable for 3D visualization and fly dissection. The system is equipped with:
- A cold halogen light source (KL 1500 LCD) for intensive illumination with infrared-free light in brightfield;
- Two LEDs for observation of fluorescent probes: GFP and RFP or similar fluorophores;
- Digitization is possible using the AxioCam ICc3 CCD color camera for brightfield or monochrome image acquisition.
Leica M205C, Brightfield stereo microscope (fly dissection) - The Leica M205C is a brightfield-only stereo microscope suitable for 3D visualization through the eyepiece and fly dissection under incident light. The system is equipped with:
- An LED cold light source for intensive illumination with infrared-free light. If you need a fluorescence stereoscope for fly dissection, see our Zeiss Discovery.V8 instead.
